This Guide was originally published in 2001 as A Practical Guide to Containment: Greenhouse Research with Transgenic Plants and Microbes and primarily addressed containment of plants and plant-associated organisms containing recombinant DNA (rDNA). Researchers, facility managers, and regulators have subsequently encouraged the authors to expand this Guide beyond containment of solely transgenic organisms. Therefore, the reader will find new information on containment strategies for research with exotics (non-native invasive species), pathogens, insects, and genetically engineered (GE) plant-manufactured pharmaceuticals and industrial compounds, and on high containment for quarantined organisms, including those on the Select Agent list.