The Training Manual on Biosafety of Genetically Modified Organism (GMOs) is prepared based on Cartagena Protocol on Biosafety, Biosafety Guidelines of Bangladesh, Biosafety Rules of Bangladesh, Guidelines for the Environmental Risk Assessment (ERA) of Genetically Engineered Plants, Guidelines for the Safety Assessment of Foods Derived from Genetically Engineered Plants and National Biosafety Framework. The Training Manual constitutes a conceptual guide for trainers that can be used to lead them through the issues biosafety and experiences.